Dataset Submitted

Petroleum Source Identification in Marine Invertebrates

Authors: Isabel C. Romero
Identified On: Feb 20 2024 15:04 UTC
DOI: https://doi.org/10.7266/81xbk4kc
UDI: NO.x959.000:0018
Project Information

Funded By:
National Oceanic and Atmospheric Administration

Research Group:
DEEPEND/RESTORE Trends and drivers of faunal abundance of the offshore Gulf of Mexico

Point of Contact

Isabel C. Romero
University of South Florida / College of Marine Science
isabelromero@usf.edu

Data Collection Period
2011-01-10
to
2019-11-13
Theme keywords

Oil pollution

Abstract:

The aim of this study is to fingerprint crude oil in marine invertebrates using gas-chromatography with tandem mass spectrometry, operated in multiple reaction monitoring mode (GC-MS/MS/MRM). The method was tested in a controlled microcosm laboratory experiment on the marine amphipod Corophium volutator and gastropod Hydrobia ulvae. This controlled microcosm experiment provided fingerprinting diagnostic ratios that were applied to the coastal gastropod Littoraria irrorata and the deep-pelagic shrimps Acanthephyra purpurea and Systellaspis debilis.

Suggested Citation:

Isabel C. Romero. Petroleum Source Identification in Marine Invertebrates. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/81xbk4kc

Purpose:

Fingerprinting crude oil in marine invertebrates

Data Parameters and Units:

ng/g, dw

Methods:

Samples were analyzed on an Agilent 7890B gas chromatograph 7010 triple quadrupole mass spectrometer. Injections (1 µl) into the multi-mode inlet in split-less mode were conducted. Inlet temperature held at 325°C. A 30m fused silica column (Rxi 5Sil MS with Integra-Guard, 30m x 0.25mm x 0.25μm, Cat# 13623-124, Restek, Bellafonte, PA, USA) was used with the following oven parameters: initial temperature 60°C, hold time 2 min; ramp 1 rate 8°C/min to 200°C, hold time 0 min; ramp 2 rate 4°C/min to 300°C, hold time 0 min, ramp 3 rate 10°C/min to 350°C, hold time 5 min. Helium was used for carrier flow at a rate of 1 ml/min and Nitrogen gas was used for collision cell gas. The transfer line between the GC oven and the MS was held at 320°C, the ion source was held at 270°C, and both quadrupoles were held at 150°C. For accuracy and precision of sample sets, the instrument was tuned daily to PFTBA (perfluorotributylamine), and samples were checked with certified standards. Sample batches were re-analyzed when replicated standards exceeded ±20% of relative standard deviation (RSD), and/or when recoveries were low.