Dataset Submitted

Method development for quantifying simultaneously 250 intermediate- and semi-volatile organic chemicals in environmental samples

Authors: Isabel C. Romero
Identified On: Feb 20 2024 15:01 UTC
DOI: https://doi.org/10.7266/at0vh9eb
UDI: NO.x959.000:0017
Project Information

Funded By:
National Oceanic and Atmospheric Administration

Research Group:
DEEPEND/RESTORE Trends and drivers of faunal abundance of the offshore Gulf of Mexico

Point of Contact

Isabel C. Romero
University of South Florida / College of Marine Science
isabelromero@usf.edu

Data Collection Period
2019-05-20
to
2020-05-20
Theme keywords

Chemical Methods

Abstract:

A method for gas chromatography tandem mass spectrometry (GC-MS/MS) in selected reaction monitoring (SRM) mode has been developed to quantify 250 compounds of a variety of compound classes (polycyclic aromatic hydrocarbons, oxidized PAHs, organochlorinated pesticides, polychlorinated biphenyls, hopanes, steranes, tri-aromatic steroids, fecal sterols, aliphatic hydrocarbons, and plastic additives). This method was validated based on available QA/QC standards using standard reference materials.

Suggested Citation:

Isabel C. Romero. Method development for quantifying simultaneously 250 intermediate- and semi-volatile organic chemicals in environmental samples. Distributed by: GRIIDC, Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/at0vh9eb

Purpose:

To improve analytical procedures

Data Parameters and Units:

ng/g

Methods:

Samples were analyzed on an Agilent 7890B gas chromatograph 7010 triple quadrupole mass spectrometer. Injections (1 µl) into the multi-mode inlet in split-less mode were conducted. Inlet temperature held at 325°C. A 30m fused silica column (Rxi 5Sil MS with Integra-Guard, 30m x 0.25mm x 0.25μm, Cat# 13623-124, Restek, Bellafonte, PA, USA) was used with the following oven parameters: initial temperature 60°C, hold time 2 min; ramp 1 rate 8°C/min to 200°C, hold time 0 min; ramp 2 rate 4°C/min to 300°C, hold time 0 min, ramp 3 rate 10°C/min to 350°C, hold time 5 min. Helium was used for carrier flow at a rate of 1 ml/min and Nitrogen gas was used for collision cell gas. The transfer line between the GC oven and the MS was held at 320°C, the ion source was held at 270°C, and both quadrupoles were held at 150°C. For accuracy and precision of sample sets, the instrument was tuned daily to PFTBA (perfluorotributylamine), and samples were checked with certified standards. Sample batches were re-analyzed when replicated standards exceeded ±20% of relative standard deviation (RSD), and/or when recoveries were low.